DNA amplification — in vitro method?
Cell-free, rapid DNA copying technique.
PCR — main process steps?
Denaturation, annealing, extension.
Primer design — importance?
Ensures specificity and efficiency.
qPCR — detection method?
Fluorescent probes monitor amplification.
DNA cloning — process?
Transfer DNA into cells for proliferation.
Non-selective amplification — purpose?
Amplifies all DNA fragments without targeting.
PCR data analysis — method?
Real-time fluorescence during exponential phase.
LAMP — temperature?
Constant, around 60-65°C.
DNA polymerase — role?
Synthesizes new DNA strands.
PCR — advantage?
Fast, specific, high throughput.
PCR — limitation?
Error rate, size limits, contamination risk.
Taq polymerase — source?
From Thermus aquaticus bacteria.
Taq — proofreading?
Lacks 3' to 5' exonuclease activity.
Fidelity enzyme — alternative?
Archaeal polymerases with proofreading.
PCR — main advantage?
Rapid, sensitive DNA amplification.
PCR — main limitation?
Error rate and contamination risk.
DNA cloning — large fragment?
Up to 1MB in bacteria.
Non-selective amplification — key tool?
Adaptor oligonucleotides with universal sequences.
qPCR — quantification?
Absolute via standard curve; relative via ΔΔCt.
LAMP — primer count?
4 to 6 primers recognizing multiple regions.
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1. What is DNA amplification technique PCR primarily classified as?
2. What temperature is typically used during the denaturation step of the PCR cycle?
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